Semaphorin-3C (Sema3c), recombinant mouse

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 21-751aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: GSSQPQARVY LTFDELRETK TSEYFSLSHQ QLDYRILLMD EDQDRIYVGS KDHILSLNIN NISQEPLSVF WPASTIKVEE CKMAGKDPTH GCGNFVRVIQ TFNRTHLYVC GSGAFSPVCT YLNRGRRSED QVFMIDSKCE SGKGRCSFNP NVNTVSVMIN EELFSGMYID FMGTDAAIFR SLTKRNAVRT DQHNSKWLSE PMFVDAHVIP DGTDPNDAKV YFFFKERLTD NNRSTKQIHS MIARICPNDT GGQRSLVNKW TTFLKARLVC SVTDEDGPET HFDELEDVFL LETDNPRTTL VYGIFTTSSS VFKGSAVCVY HLSDIQTVFN GPFAHKEGPN HQLISYQGRI PYPRPGTCPG GAFTPNMRTT KDFPDDVVTF IRNHPLMYNS IYPIHRRPLI VRIGTDYKYT KIAVDRVNAA DGRYHVLFLG TDRGTVQKVV VLPTNSSASG ELILEELEVF KNHVPITTMK ISSKKQQLYV SSNEGVSQVS LHRCHIYGTA CADCCLARDP YCAWDGHSCS RFYPTGKRRS RRQDVRHGNP LTQCRGFNLK AYRNAAEIVQ YGVRNNSTFL ECAPKSPQAS IKWLLQKDKD RRKEVKLNER IIATSQGLLI RSVQDSDQGL YHCIATENSF KQTIAKINFK VLDSEMVAVV TDKWSPWTWA GSVRALPFHP KDILGAFSHS EMQLINQYCK DTRQQQQLGE EPQKMRGDYG KLKALINSRK SRNRRNQLPE S. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance. Reference: n/a. Function: nan