Selenoprotein M (SELENOM), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 24-145aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: ATAYRPDWNR LSGLTRARVE TCGGSQLNRL KEVKAFVTQD IPFYHNLVMK HLPGADPELV LLGRRYEELE RIPLSEMTRE EINALVQELG FYRKAAPDAQ VPPEYVWAPA KPPEETSDHA DL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May function as a thiol-disulfide oxidoreductase that participates in disulfide bond formation. Reference: "Mammalian selenoprotein in which selenocysteine (Sec) incorporation is supported by a new form of Sec insertion sequence element." Korotkov K.V., Novoselov S.V., Hatfield D.L., Gladyshev V.N. Mol. Cell. Biol. 22:1402-1411(2002). Function: May function as a thiol-disulfide oxidoreductase that participates in disulfide bond formation.