Secretogranin-3 (Scg3), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 23-471aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: FPKPEGSQDK SLHNRELSAE RPLNEQIAEA EADKIKKTYP SESKPSESNF SSVDNLNLLK AITEKETVEK AKQSIRSSPF DNRLNVDDAD STKNRKLTDE YDSTKSGLDR KVQDDPDGLH QLDGTPLTAE DIVHKIATRI YEENDRGVFD KIVSKLLNLG LITESQAHTL EDEVAEALQK LISKEANNYE EAPEKPTSRT ENQDGKIPEK VTPVAATQDG FTNRENDDTV SNTLTLSNGL ERRTNPHRDD DFEELQYFPN FYALLTSIDS EKEAKEKETL ITIMKTLIDF VKMMVKYGTI SPEEGVSYLE NLDETIALQT KNKLEKNTTD SKSKLFPAPP EKSHEETDST KEEAAKMEKE YGSLKDSTKD DNSNLGGKTD EAKGKTEAYL EAIRKNIEWL KKHNKKGNKE DYDLSKMRDF INQQADAYVE KGILDKEEAN AIKRIYSSL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:12388744, PubMed:14597614). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (PubMed:12388744, PubMed:14597614). May also play a role in angiogenesis. Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (By similarity). Reference: "Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A." Hosaka M., Suda M., Sakai Y., Izumi T., Watanabe T., Takeuchi T. J. Biol. Chem. 279:3627-3634(2004). Function: nan