Schlafen family member 12 (SLFN12), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-578aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MNISVDLETN YAELVLDVGR VTLGENSRKK MKDCKLRKKQ NESVSRAMCA LLNSGGGVIK AEIENEDYSY TKDGIGLDLE NSFSNILLFV PEYLDFMQNG NYFLIFVKSW SLNTSGLRIT TLSSNLYKRD ITSAKVMNAT AALEFLKDMK KTRGRLYLRP ELLAKRPCVD IQEENNMKAL AGVFFDRTEL DRKEKLTFTE STHVEIKNFS TEKLLQRIKE ILPQYVSAFA NTDGGYLFIG LNEDKEIIGF KAEMSDLDDL EREIEKSIRK MPVHHFCMEK KKINYSCKFL GVYDKGSLCG YVCALRVERF CCAVFAKEPD SWHVKDNRVM QLTRKEWIQF MVEAEPKFSS SYEEVISQIN TSLPAPHSWP LLEWQRQRHH CPGLSGRITY TPENLCRKLF LQHEGLKQLI CEEMDSVRKG SLIFSRSWSV DLGLQENHKV LCDALLISQD SPPVLYTFHM VQDEEFKGYS TQTALTLKQK LAKIGGYTKK VCVMTKIFYL SPEGMTSCQY DLRSQVIYPE SYYFTRRKYL LKALFKALKR LKSLRDQFSF AENLYQIIGI DCFQKNDKKM FKSCRRLT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. Reference: "A Y2H-seq approach defines the human protein methyltransferase interactome." Weimann M., Grossmann A., Woodsmith J., Ozkan Z., Birth P., Meierhofer D., Benlasfer N., Valovka T., Timmermann B., Wanker E.E., Sauer S., Stelzl U. Nat. Methods 10:339-342(2013). Function: nan