ScaI

5'-A G T^A C T-3' , 3'-T C A^T G A-5', Concentration: 10u/ul Source: Streptomyces caespitosus, Supplied with: R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X 37mM Tris-acetate (pH 7.0), 15mM magnesium acetate, 150mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C, Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. , Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.