SARS-CoV-2 nsp8 (recombinant)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. SARS-CoV-2 non-structural protein 8 (nsp8) is encoded within ORF1ab and is a cofactor and component of the SARS-CoV-2 decameric RNA replication transcriptional complex. It functions as an anchoring scaffold for the RNA transcription complex, with its N-terminal domain (NTD) binding to the RNA-dependent polymerase (RdRp) nsp12, the helicase nsp13, and RNA. SARS-CoV-2 nsp8 also functions as a virulence factor, binding to the host 7SL RNA component of the signal recognition particle to suppress membrane protein trafficking and impair host antiviral responses.Synonyms: Severe Acute Respiratory Syndrome Coronavirus 2 nsp8, SARS-CoV-2 Non-structural Protein 8. Purity: >90% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant SARS-CoV-2 N-terminal His- and DYKDDK-tagged nsp8 expressed in E. coli. Amino Acids: 1-198. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride and 20% glycerol. UniProt Accession #: P0DTD1.