SARS-CoV-2 nsp1 (recombinant)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. SARS-CoV-2 non-structural protein 1 (nsp1) is a multifunctional protein and virulence factor that inhibits host mRNA translation, including translation of antiviral proteins such as IFN-beta and IFN-lambda1. It is composed of an N-terminal domain and a C-terminal domain connected via an unstructured 20-amino acid residue linker. The SARS-CoV-2 nsp1 C-terminal domain binds to the mRNA entry site of the 40S subunit of the host ribosome, which prevents host mRNA from entering, while the N-terminal binds to the 40S decoding center preventing accurate alignment of tRNA and mRNA. SARS-CoV-2 nsp1 also induces host mRNA degradation, prevents mRNA export from the nucleus to the cytoplasm, and binds to pre-rRNA in the nucleolus to disrupt rRNA biogenesis.Synonyms: SARS-CoV-2 Host Translation Inhibitor nsp1, SARS-CoV-2 Non-structural Protein 1, Severe Acute Respiratory Syndrome Coronavirus 2 nsp1. Purity: >90%. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant SARS-CoV-2 N-terminal His-tagged nsp1 expressed in E. coli. Amino Acids: 13-128. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol. UniProt Accession #: P0DTD1.