Saporin (Saponaria officinalis, Common Soapwort, ribosome inactivating protein saporin-6, SAP-6, SO-

Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. Source: Purified from seeds of the plant Saponaria officinalis. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Biological Activity: Upon introduction into the cells, saporin will arrest the protein synthesis by damaging the ribosmes. Handling: Only qualified personnel should handle this product. Extreme precaution must be employed when handling the toxin. Wear lab coat, gloves and safety glasses. The toxin must not come into contact with open wounds. Practice good laboratory technique to avoid exposure/auto-injection! Wash thoroughly any part of the body exposed to the toxin. Contaminated materials should be exposed to 300mM NaOH or autoclaved. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.