Roundabout homolog 3 (ROBO3), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 64-531aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: PRIVEQPPDL LVSRGEPATL PCRAEGRPRP NIEWYKNGAR VATVREDPRA HRLLLPSGAL FFPRIVHGRR ARPDEGVYTC VARNYLGAAA SRNASLEVAV LRDDFRQSPG NVVVAVGEPA VLECVPPRGH PEPSVSWRKD GARLKEEEGR ITIRGGKLMM SHTLKSDAGM YVCVASNMAG ERESAAAEVM VLERPSFLRR PVNQVVLADA PVTFLCEVKG DPPPRLRWRK EDGELPTGRY EIRSDHSLWI GHVSAEDEGT YTCVAENSVG RAEASGSLSV HVPPQLVTQP QDQMAAPGES VAFQCETKGN PPPAIFWQKE GSQVLLFPSQ SLQPTGRFSV SPRGQLNITA VQRGDAGYYV CQAVSVAGSI LAKALLEIKG ASLDGLPPVI LQGPANQTLV LGSSVWLPCR VTGNPQPSVR WKKDGQWLQG DDLQFKTMAN GTLYIANVQE MDMGFYSCVA KSSTGEAT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Receptor involved in axon guidance during development. Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself. Reference: n/a. Function: nan