RNA binding protein fox-1 homolog 3 (Rbfox3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-374aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MAQPYPPAQY PPPPQNGIPA EYAPPPPHPT QDYSGQTPVP PEHGMTLYTP AQTHPEQPGT EASTQPIAGT QTVPQADEAA QTDNQQLHPS DPTEKQQPKR LHVSNIPFRF RDPDLRQMFG QFGKILDVEI IFNERGSKGF GFVTFETSSD ADRAREKLNG TIVEGRKIEV NNATARVMTN KKPGNPYANG WKLNPVVGTV YGPEFYAVTS FPYPTTGTAV AYRGAHLRGR GRAVYNTFRA APPPPPIPTY GAALEQTLVK MPVPWAGLAP CPLPPQQTPE PAYPTSPAFP PLSCPFASRV VYQDGFYGAE IYGGYAAYRY AQPAAATAAA YSDSYGRVYA AADPYHHTIG PTATYSIGTM ASLCRGGYSR FTPY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). Reference: "NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2." Dredge B.K., Jensen K.B. PLoS ONE 6:E21585-E21585(2011). Function: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).