Ribulose bisphosphate carboxylase large chain (rbcL), Oryza sativa subsp. japonica, recombinant

Organism: Oryza sativa subsp. japonica (Rice). Source: E.coli. Expression Region: 3-477aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: PQTETKASVG FKAGVKDYKL TYYTPEYETK DTDILAAFRV TPQPGVPPEE AGAAVAAESS TGTWTTVWTD GLTSLDRYKG RCYHIEPVVG EDNQYIAYVA YPLDLFEEGS VTNMFTSIVG NVFGFKALRA LRLEDLRIPP TYSKTFQGPP HGIQVERDKL NKYGRPLLGC TIKPKLGLSA KNYGRACYEC LRGGLDFTKD DENVNSQPFM RWRDRFVFCA EAIYKSQAET GEIKGHYLNA TAGTCEEMIK RAVFARELGV PIVMHDYLTG GFTANTSLAH YCRDNGLLLH IHRAMHAVID RQKNHGMHFR VLAKALRMSG GDHIHAGTVV GKLEGEREMT LGFVDLLRDD FIEKDRARGI FFTQDWVSMP GVIPVASGGI HVWHMPALTE IFGDDSVLQF GGGTLGHPWG NAPGAAANRV ALEACVQARN EGRDLAREGN EIIRSACKWS PELAAACEIW KAIKFEFEPV DKLDS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Reference: "Crystal structure of rice Rubisco and implications for activation induced by positive effectors NADPH and 6-phosphogluconate." Matsumura H., Mizohata E., Ishida H., Kogami A., Ueno T., Makino A., Inoue T., Yokota A., Mae T., Kai Y. J. Mol. Biol. 422:75-86(2012). Function: nan