Ribulose bisphosphate carboxylase large chain (rbcL), Glycine max, recombinant

Organism: Glycine max (Soybean) (Glycine hispida). Source: E.coli. Expression Region: 3-475aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: PQTETKASVG FKAGVKDYKL TYYTPDYETK DTDILAAFRV TPQPGVPPEE AGAAVAAESS TGTWTTVWTD GLTSLDRYKG RCYGLEPVAG EENQYIAYVA YPLDLFEEGS VTNMFTSIVG NVFGFKALRA LRLEDLRIPT AYIKTFQGPP HGIQVERDKL NKYGRPLLGC TIKPKLGLSA KNYGRAVYEC LRGGLDFTKD DENVNSQPFM RWRDRFLFCA EAIFKSQAET GEIKGHYLNA TAGTCEEMMK RAVFARELGV PIVMHDYLTG GFTANTSLAH YCRDNGLLLH IHRAMHAVID RQKNHGMHFR VLAKALRLSG GDHVHAGTVV GKLEGEREIT LGFVDLLRDD FVEKDRSRGI YFTQDWVSLP GVLPVASGGI HVWHMPALTE IFGDDSVLQF GGGTLGHPWG NAPGAVANRV ALEACVQARN EGRDLAREGN EIIREASKWS PELAAACEVW KEIKFEFEAM DTL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Reference: "Complete chloroplast genome sequence of Glycine max and comparative analyses with other legume genomes."Saski C., Lee S.-B., Daniell H., Wood T.C., Tomkins J., Kim H.-G., Jansen R.K.Plant Mol. Biol. 59:309-322(2005). Function: RuBisCO catalyzes two reactions