Rho guanine nucleotide exchange factor 12 (ARHGEF12), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 367-558aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: GQCSCFQSIE LLKSRPAHLA VFLHHVVSQF DPATLLCYLY SDLYKHTNSK ETRRIFLEFH QFFLDRSAHL KVSVPDEMSA DLEKRRPELI PEDLHRHYIQ TMQERVHPEV QRHLEDFRQK RSMGLTLAES ELTKLDAERD KDRLTLEKER TCAEQIVAKI EEVLMTAQAV EEDKSSTMQY VILMYMKHLG VK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Reference: "Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor." Kristelly R., Gao G., Tesmer J.J. J. Biol. Chem. 279:47352-47362(2004). Function: nan