Rhamnogalacturonan endolyase 4A, Dickeya dadantii

Rhamnogalacturonan endolyase 4A (DdRge4A), assigned the E.C. number 4.2.2.23, is a derivative of Dickeya dadantii. It is an enzyme that participates in the endotype eliminative cleavage of L-alpha-rhamnopyranosyl-1,4-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant DdRge4A, purified from Escherichia coli, is a single-domain Pectate Lyase family 4 (PL4) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Pectate Lyase. Activity: Rhamnogalacturonan lyases. Family: PL4. Ec Number: 4.2.2.23. Organism: Dickeya dadantii. Architecture: PL4. Optimal pH: 6. Optimal Temperature: 37°C. Substrates: rhamnogalacturonans