Retinoid isomerohydrolase (RPE65), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 2-533aa. Protein Length: Full Length. Tag Info: Tag-Free. Target Protein Sequence: SIQVEHPAGG YKKLFETVEE LSSPLTAHVT GRIPLWLTGS LLRCGPGLFE VGSEPFYHLF DGQALLHKFD FKEGHVTYHR RFIRTDAYVR AMTEKRIVIT EFGTCAFPDP CKNIFSRFFS YFRGVEVTDN ALVNVYPVGE DYYACTETNF ITKINPETLE TIKQVDLCNY VSVNGATAHP HIENDGTVYN IGNCFGKNFS IAYNIVKIPP LQADKEDPIS KSEIVVQFPC SDRFKPSYVH SFGLTPNYIV FVETPVKINL FKFLSSWSLW GANYMDCFES NETMGVWLHI ADKKRKKYLN NKYRTSPFNL FHHINTYEDN GFLIVDLCCW KGFEFVYNYL YLANLRENWE EVKKNARKAP QPEVRRYVLP LNIDKADTGK NLVTLPNTTA TAILCSDETI WLEPEVLFSG PRQAFEFPQI NYQKYCGKPY TYAYGLGLNH FVPDRLCKLN VKTKETWVWQ EPDSYPSEPI FVSHPDALEE DDGVVLSVVV SPGAGQKPAY LLILNAKDLS EVARAEVEIN IPVTFHGLFK KS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore . Essential for the production of 11-cis retinal for both rod and cone photoreceptors . Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid . The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. Reference: "RPE65 has an additional function as the lutein to meso-zeaxanthin isomerase in the vertebrate eye." Shyam R., Gorusupudi A., Nelson K., Horvath M.P., Bernstein P.S. Proc. Natl. Acad. Sci. U.S.A. 114:10882-10887(2017). Function: nan