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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
R1665.1 | 1 kit | - | - |
3 - 19 business days* |
369.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling... more
Product information "Retinoblastoma, Recombinant Fusion Protein, aa701-928, Negative and Positive Control (Rb)"
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by cdk's inhibits Rb target binding, thus allowing cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (6). Specificity of different cdk/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). R1665A Rb-C Fusion Protein Non-Phosphorylated 1x100ul Rb-C is expressed as a recombinant fusion protein of Rb residues 701-928 and maltose binding protein, serves as a negative control. R1665BRb-C Fusion Protein Phosphorylated: 1x100ul Prepared by in vitro kinase reaction with cdc2, serves as a positive control. Application:, Suitable for use as a control in Western Blot. Recommended Dilution: 10ul (50ng), Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Rb, RB1, pRb, pp110, p105-Rb, Retinoblastoma-associated protein |
Supplier: | United States Biological |
Supplier-Nr: | R1665 |
Properties
Application: | WB |
Conjugate: | No |
Database Information
KEGG ID : | K06618 | Matching products |
UniProt ID : | P06400 | Matching products |
Gene ID | GeneID 5925 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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