Retina-specific copper amine oxidase (AOC2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 33-756aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: QPPHCPSVSH RAQPWPHPGQ SQLFADLSRE ELTAVMRFLT QRLGPGLVDA AQAQPSDNCI FSVELQLPPK AAALAHLDRG SPPPAREALA IVLFGGQPQP NVSELVVGPL PHPSYMRDVT VERHGGPLPY HRRPVLRAEF TQMWRHLKEV ELPKAPIFLS STFNYNGSTL AAVHATPRGL RSGDRATWMA LYHNISGVGL FLHPVGLELL LDHRALDPAH WTVQQVFYLG HYYADLGQLE REFKSGRLEV VRVPLPPPNG ASSLRSRNSP GPLPPLQFSP QGSQYSVQGN LVVSSLWSFT FGHGVFSGLR IFDVRFQGER IAYEVSVQEC VSIYGADSPK TMLTRYLDSS FGLGRNSRGL VRGVDCPYQA TMVDIHILVG KGAVQLLPGA VCVFEEAQGL PLRRHHNYLQ NHFYGGLASS ALVVRSVSSV GNYDYIWDFV LYPNGALEGR VHATGYINTA FLKGGEEGLL FGNRVGERVL GTVHTHAFHF KLDLDVAGLK NWVVAEDVVF KPVAAPWNPE HWLQRPQLTR QVLGKEDLTA FSLGSPLPRY LYLASNQTNA WGHQRGYRIQ IHSPLGIHIP LESDMERALS WGRYQLVVTQ RKEEESQSSS IYHQNDIWTP TVTFADFINN ETLLGEDLVA WVTASFLHIP HAEDIPNTVT LGNRVGFLLR PYNFFDEDPS IFSPGSVYFE KGQDAGLCSI NPVACLPDLA ACVPDLPPFS YHGF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina. Reference: "Human retina-specific amine oxidase: genomic structure of the gene (AOC2), alternatively spliced variant, and mRNA expression in retina." Imamura Y., Noda S., Mashima Y., Kudoh J., Oguchi Y., Shimizu N. Genomics 51:293-298(1998). Function: Has a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina.