Reticulon-3 (Rtn3), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 182-440aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: KDQEPKNPNK VPDGEDRSAL DFGQSKAEHI CTYSLSPSEL PVASVEKDSP ESPFEVIIDK ATFDREFKDL YKENPNDLGG WAAHGDRESP ADLLEMNDKL FPLRNKEAGR YPSSVLLGRQ FSHTTAALEE VSRCVNDMHN FTNEILTWDL DPQAKQQANK TSCTTESTGL DRSELRSEIP VINLKTNPQQ KMPVCSFNGS TPITKSTGDW TEAFTEGKPV RDYLSSTKEA GGNGVPGSSQ LHSELPGSMP EKWVSGSGA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress . Induces the formation of endoplasmic reticulum tubules. Acts also as an inflammation-resolving regulator by interacting with both TRIM25 and RIG-I/DDX58, subsequently impairing DDX58 'Lys-63'-linked polyubiquitination leading to IRF3 and NF-kappa-B inhibition . Reference: "Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion." Yamamoto Y., Yoshida A., Miyazaki N., Iwasaki K., Sakisaka T. Biochem. J. 458:69-79(2014). Function: nan