Recombinant SMARCAL1 Enzyme, Active, Human

Active, human SMARCAL1 (AA 226-870) protein expressed and purified from baculovirus-infected insect cells with an N-Terminal 6xHis tag. The enzyme has been thoroughly validated with the Transcreener ADP2 Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is 95% in a composition of 50 mM HEPES, 100 mM NaCl, 10% Glycerol, 1 mM TCEP, pH 7.5. Protein Function: ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks [The Uniprot Consortium]