Recombinant Human DTD2 Protein

Tag: C-His. Modification: unmodified. Start Site: Val61. End Site: Gly160. Coverage: 0.69. Isoelectric Point: 7.5. Core Sequence: VNTLLNVKLS ETENGKHVSI LDLPGNILII PQATLGGRLK GRNMQYHSNS GKEEGFELYS QFVTLCEKEV AANSKCAEAR VVVEHGTYGN RQVLKLDTNG. Homologies: Highest protein sequence identity to the following orthologs: Mouse - 84%, Pig - 92%, Cynomolgus monkey - 97%, Protein Function: Deacylates mischarged D-aminoacyl-tRNAs (By similarity). Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity). Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids (By similarity). Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr) (By similarity). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS (PubMed:29410408). Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408). Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation (By similarity) [The Uniprot Consortium]