Recombinant Human CHST14 Protein

Tag: N-His. Modification: unmodified. Start Site: Leu71. End Site: Thr370. Coverage: 0.84. Isoelectric Point: 9. Core Sequence: LPLHPPGREG TAWRGKAPKP GGLSLRAGDA DLQVRQDVRN RTLRAVCGQP GMPRDPWDLP VGQRRTLLRH ILVSDRYRFL YCYVPKVACS NWKRVMKVLA GVLDSVDVRL KMDHRSDLVF LADLRPEEIR YRLQHYFKFL FVREPLERLL SAYRNKFGEI REYQQRYGAE IVRRYRAGAG PSPAGDDVTF PEFLRYLVDE DPERMNEHWM PVYHLCQPCA VHYDFVGSYE RLEADANQVL EWVRAPPHVR FPARQAWYRP ASPESLHYHL CSAPRALLQD VLPKYILDFS LFAYPLPNVT. Homologies: Highest protein sequence identity to the following orthologs: Mouse - 93%, Rat - 39%, Pig - 94%, Cynomolgus monkey - 99%, Protein Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development [The Uniprot Consortium]