Ras-related protein Rab-27B (RAB27B), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-218aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: TDGDYDYLIK LLALGDSGVG KTTFLYRYTD NKFNPKFITT VGIDFREKRV VYNAQGPNGS SGKAFKVHLQ LWDTAGQERF RSLTTAFFRD AMGFLLMFDL TSQQSFLNVR NWMSQLQANA YCENPDIVLI GNKADLPDQR EVNERQAREL ADKYGIPYFE TSAATGQNVE KAVETLLDLI MKRMEQCVEK TQIPDTVNGG NSGNLDGEKP PEKKCIC. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in targeting uroplakins to urothelial apical mbranes. Reference: Molecular cloning and characterization of rab27a and rab27b, novel human rab proteins shared by melanocytes and platelets.Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.Biochem. Mol. Med. 60:27-37(1997). Function: May be involved in targeting uroplakins to urothelial apical membranes.