Ras association domain-containing protein 6 (RASSF6), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-303aa. Protein Length: Full Length of Isoform 4. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MTMMAHQYPS WIFINEKTFI TREQLNSLLK TYNIFYENQK NLHILYGETE DGKLIVEGML DIFWGVKRPI QLKIQDEKPF SSFTSMKSSD VFSSKGMTRW GEFDDLYRIS ELDRTQIPMS EKRNSQEDYL SYHSNTLKPH AKDEPDSPVL YRTMSEAALV RKRMKPLMMD RKERQKNRAS INGHFYNHEI ENSPQDFALH IIFATGEQRR LKKTDIPLLQ RLLQGPSEKN ARIFLMDKDA EEISSDVAQY INFHFSLLES ILQRLNEEEK REIQRIVTKF NKEKAIILKC LQNKLVIKTE TTV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in the induction of apoptosis, through both caspase-dependent and caspase-independent pathways. May act as a Ras effector protein. May suppress the serum-induced basal levels of NF-kappa-B. Reference: "RASSF6, a member of the RASSF family of putative tumor suppressors, is transcriptionally repressed in lung and breast cancers." Burbee D.G., White M.A., Miller D.S., Minna J.D., Muller C.Y. Submitted (JAN-2003). Function: Involved in the induction of apoptosis, through both caspase-dependent and caspase-independent pathways. May act as a Ras effector protein. May suppress the serum-induced basal levels of NF-kappa-B (By similarity).