Ral BP1, Recombinant, Rat (Ral Assay Reagent) (Agarose), Purified

Source: , GST-tagged fusion protein corresponding to residues 397-519 of rat Ral binding protein, expressed in E. coli , Applications: Af?nity Precipitation: 10-15ul (5-7.5ug) of a 50% slurry precipitated RalAGTP and RalB-GTP from 1mg of EGF-stimulated Cos-7 cells which had been lysed in a magnesium containing lysis buffer. , Note: Due to spontaneous deactivation of RalA and RalB, use lysates that have been stored at-70°C for less than 1 month. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.