Rab5 GDP/GTP exchange factor (RABGEF1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-491aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MSLKSERRGI HVDQSDLLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQKQ IQEDWELAER LQREEEEAFA SSQSSQGAQS LTFSKFEEKK TNEKTRKVTT VKKFFSASSR VGSKKEIQEA KAPSPSINRQ TSIETDRVSK EFIEFLKTFH KTGQEIYKQT KLFLEGMHYK RDLSIEEQSE CAQDFYHNVA ERMQTRGKVP PERVEKIMDQ IEKYIMTRLY KYVFCPETTD DEKKDLAIQK RIRALRWVTP QMLCVPVNED IPEVSDMVVK AITDIIEMDS KRVPRDKLAC ITKCSKHIFN AIKITKNEPA SADDFLPTLI YIVLKGNPPR LQSNIQYITR FCNPSRLMTG EDGYYFTNLC CAVAFIEKLD AQSLNLSQED FDRYMSGQTS PRKQEAESWS PDACLGVKQM YKNLDLLSQL NERQERIMNE AKKLEKDLID WTDGIAREVQ DIVEKYPLEI KPPNQPLAAI DSENVENDKL PPPLQPQVYA G. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic mbrane fusion and mbrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase . Reference: Seven genes that are differentially transcribed in colorectal tumor cell lines.Nimmrich I., Erdmann S., Melchers U., Finke U., Hentsch S., Moyer M.P., Hoffmann I., Mueller O.Cancer Lett. 160:37-43(2000). Function: Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity).