Pyruvate dehydrogenase protein X component, mitochondrial (PDHX), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 54-501aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GDPIKILMPS LSPTMEEGNI VKWLKKEGEA VSAGDALCEI ETDKAVVTLD ASDDGILAKI VVEEGSKNIR LGSLIGLIVE EGEDWKHVEI PKDVGPPPPV SKPSEPRPSP EPQISIPVKK EHIPGTLRFR LSPAARNILE KHSLDASQGT ATGPRGIFTK EDALKLVQLK QTGKITESRP TPAPTATPTA PSPLQATAGP SYPRPVIPPV STPGQPNAVG TFTEIPASNI RRVIAKRLTE SKSTVPHAYA TADCDLGAVL KVRQDLVKDD IKVSVNDFII KAAAVTLKQM PDVNVSWDGE GPKQLPFIDI SVAVATDKGL LTPIIKDAAA KGIQEIADSV KALSKKARDG KLLPEEYQGG SFSISNLGMF GIDEFTAVIN PPQACILAVG RFRPVLKLTE DEEGNAKLQQ RQLITVTMSS DSRVVDDELA TRFLKSFKAN LENPIRLA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Reference: "Characterization of interactions of dihydrolipoamide dehydrogenase with its binding protein in the human pyruvate dehydrogenase complex." Park Y.H., Patel M.S. Biochem. Biophys. Res. Commun. 395:416-419(2010). Function: nan