Puromycin-sensitive aminopeptidase (NPEPPS), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 53-504aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RLPADVSPIN YSLCLKPDLL DFTFEGKLEA AAQVRQATNQ IVMNCADIDI ITASYAPEGD EEIHATGFNY QNEDEKVTLS FPSTLQTGTG TLKIDFVGEL NDKMKGFYRS KYTTPSGEVR YAAVTQFEAT DARRAFPCWD EPAIKATFDI SLVVPKDRVA LSNMNVIDRK PYPDDENLVE VKFARTPVMS TYLVAFVVGE YDFVETRSKD GVCVRVYTPV GKAEQGKFAL EVAAKTLPFY KDYFNVPYPL PKIDLIAIAD FAAGAMENWG LVTYRETALL IDPKNSCSSS RQWVALVVGH ELAHQWFGNL VTMEWWTHLW LNEGFASWIE YLCVDHCFPE YDIWTQFVSA DYTRAQELDA LDNSHPIEVS VGHPSEVDEI FDAISYSKGA SVIRMLHDYI GDKDFKKGMN MYLTKFQQKN AATEDLWESL ENASGKPIAA VMNTWTKQMG FP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. Reference: "Human puromycin-sensitive aminopeptidase: cloning of 3' UTR, evidence for a polymorphism at a.a. 140 and refined chromosomal localization to 17q21." Bauer W.O., Nanda I., Beck G., Schmid M., Jakob F. Cytogenet. Cell Genet. 92:221-224(2001). Function: nan