PtdIns-(3,4,5)-P3 Binding Protein

Pleckstrin homology (PH) domains all contain a seven-standard beta-sandwich that allows binding to various species of phosphatidyl inositol (PtdIns) phosphates. Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(3,4,5)-P3 Binding Protein contains a highly specific PH domain that recognizes and binds PtdIns-(3,4,5)-P3 (see Figure 1). PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.{4096, 8344} PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signalling cascade that has been implicated in cancer. Due to PtdIns-(3,4,5)-P3 binding protein · s unique affinity for PtdIns-(3,4,5)-P3, this protein can be used to detect product formed by PI3-kinase in in vitro assays. Cayman's PtdIns-(3,4,5)-P3 Binding Protein is isoform 2, which differs from isoform 1 by the absence of a glycine residue at position 277.Synonyms: Cyth3, Cytohesin 3, GRP1, GRP1 PH Domain, PIP3, PIP3[3',4',5'] Binding Protein, PI(3,4,5)-P3, PI(3,4,5)P3 Binding Protein, Phosphatidylinositol-3,4,5-triphosphate. Purity: >95%. Source: Recombinant human N-terminal GST-tagged PIP3 expressed in E. coli. Amino Acids: 264-381 (PH domain). MW: ~41.7 kDa. Formulation: (Request formulation change), A solution in PBS, pH 7.4, containing 20% glycerol. UniProt Accession #: O43739.