Protocadherin-19 (PCDH19), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 22-678aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: LINLKYSVEE EQRAGTVIAN VAKDAREAGF ALDPRQASAF RVVSNSAPHL VDINPSSGLL VTKQKIDRDL LCRQSPKCII SLEVMSSSME ICVIKVEIKD LNDNAPSFPA AQIELEISEA ASPGTRIPLD SAYDPDSGSF GVQTYELTPN ELFGLEIKTR GDGSRFAELV VEKSLDRETQ SHYSFRITAL DGGDPPRLGT VGLSIKVTDS NDNNPVFSES TYAVSVPENS PPNTPVIRLN ASDPDEGTNG QVVYSFYGYV NDRTRELFQI DPHSGLVTVT GALDYEEGHV YELDVQAKDL GPNSIPAHCK VTVSVLDTND NPPVINLLSV NSELVEVSES APPGYVIALV RVSDRDSGLN GRVQCRLLGN VPFRLQEYES FSTILVDGRL DREQHDQYNL TIQARDGGVP MLQSAKSFTV LITDENDNHP HFSKPYYQVI VQENNTPGAY LLSVSARDPD LGLNGSVSYQ IVPSQVRDMP VFTYVSINPN SGDIYALRSF NHEQTKAFEF KVLAKDGGLP SLQSNATVRV IILDVNDNTP VITAPPLING TAEVYIPRNS GIGYLVTVVK AEDYDEGENG RVTYDMTEGD RGFFEIDQVN GEVRTTRTFG ESSKSSYELI VVAHDHGKTS LSASALVLIY LSPALDAQES MGSVNLS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Potential calcium-dependent cell-adhesion protein. Reference: "Family-wide Structural and Biophysical Analysis of Binding Interactions among Non-clustered delta-Protocadherins." Harrison O.J., Brasch J., Katsamba P.S., Ahlsen G., Noble A.J., Dan H., Sampogna R.V., Potter C.S., Carragher B., Honig B., Shapiro L. Cell Rep 30:2655-2671.e7(2020). Function: nan