Protein NDNF (Ndnf), recombinant mouse

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 20-568aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: QKLPTRDEEL FQMQIRDKEF FHDSSVIPDG AEVSSYLFRD TPRRYFFMVE EDNTPLSVTV TPCDAPLEWK LSLQELHEGS SADGSGDPEL LDQQKQQMTD VEGTELFSYK GNDVEYFLSS SSPSGLYQLE LLSTEKDTHF KVYATTTPES DQPYPELPYD PRVDVTSFGR TTVTLAWKPS PTASILKQPI EYCVVINKEH NFKSLCAAET KMNADDAFMV APKPGLDFNP FDFAHFGFPT DNLGKDRSLL AKPSPKVGRH VYWRPKVDIQ KICIGNKNIF TVSDLKPDTQ YYFDVFMVNT NTNMSTAYVG AFVRTKEEAK QKTVELKDGR VTDVFVKRKG KKFLRFAPVS SHQKVTFFIH SCMDAVQVQV RRDGRLLLSQ NVEGIRQFQL RGKPKGKYLI RLKGNRKGAS KLKILATTRP SKHAFPSLPE DTRIKAFDKL RTCSSVTVAW LGTQERRKFC IYRKEVDGNY SEDQKRREQN QCLGPDTRKK SEKVLCKYFH SQNLQKAVTT ETIRDLQPGK SYLLDVYVVG HGGHSVKYQS KIVKTRKVC. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness. Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms. Reference: n/a. Function: nan