Protein mono-ADP-ribosyltransferase PARP10 (PARP10), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 650-1025aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Twin-Strep-tagged. Target Protein Sequence: LEEEAALQLA LHRSLEPQGQ VAEQEEAAAL RQALTLSLLE QPPLEAEEPP DGGTDGKAQL VVHSAFEQDV EELDRALRAA LEVHVQEETV GPWRRTLPAE LRARLERCHG VSVALRGDCT ILRGFGAHPA RAARHLVALL AGPWDQSLAF PLAASGPTLA GQTLKGPWNN LERLAENTGE FQEVVRAFYD TLDAARSSIR VVRVERVSHP LLQQQYELYR ERLLQRCERR PVEQVLYHGT TAPAVPDICA HGFNRSFCGR NATVYGKGVY FARRASLSVQ DRYSPPNADG HKAVFVARVL TGDYGQGRRG LRAPPLRGPG HVLLRYDSAV DCICQPSIFV IFHDTQALPT HLITCEHVPR ASPDDPSGLP GRSPDT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity. Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA. Reference: n/a. Function: nan