Protein HAPLESS 2 (HAP2), recombinant Arabidopsis thaliana

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: in vitro E.coli expression system. Expression Region: 25-705aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: IQILSKSKLE KCEKTSDSGN LNCSTKIVLN LAVPSGSSGG EASIVAEIVE VEDNSSSNMQ TVRIPPVITV NKSAAYALYD LTYIRDVPYK PQEYHVTTRK CEHDAGPDIV QICERLRDEK GNVLEQTQPI CCPCGPQRRM PSSCGDIFDK MIKGKANTAH CLRFPGDWFH VFGIGQRSLG FSVRVELKTG TRVSEVIIGP ENRTATANDN FLKVNLIGDF GGYTSIPSFE DFYLVIPREA AEAGQPGSLG ANYSMWMLLE RVRFTLDGLE CNKIGVGYEA FNTQPNFCSS PYWSCLHNQL WNFRESDINR IDRHQLPLYG LEGRFERINQ HPNAGPHSFS IGVTETLNTN LMIELRADDI EYVFQRSPGK IINIAIPTFE ALTQFGVAAV IIKNTGEVEA SYSLTFDCSK GVAFVEEQFF IIKPKAVTTR SFKLYPTKDQ AAKYICTAIL KDSQFSEVDR AECQFSTTAT VLDNGTQVTN PFQIPETQPK GFFDSIRILW TKIINGLVDF ITGDTCRNKC SSFFDFSCHI QYVCLSWMVM FGLLLALFPI TCLLLWLLHQ KGLFDPCYDW WEDHFDLDHH RRLLPSRADV VNRHHHHHKH RHHHNHHRRT HQRHKHHHGQ DDDVLQKMML ERDHSDSHYY HQLHRVHKDS KQKQRRRAKH GIVLPRDVHV ERQRKQRLRE S. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm. Mediates the fusion of cell membranes. Not required for pollen tube outgrowth. Reference: n/a. Function: nan