Propionyl-CoA carboxylase beta chain, mitochondrial (PCCB), recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-539aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: CSQATSVNER IENKRRTALL GGGQRRIDAQ HKRGKLTARE RISLLLDPGS FVESDMFVEH RCADFGMAAD KNKFPGDSVV TGRGRINGRL VYVFSQDFTV FGGSLSGAHA QKICKIMDQA ITVGAPVIGL NDSGGARIQE GVESLAGYAD IFLRNVTASG VIPQISLIMG PCAGGAVYSP ALTDFTFMVK DTSYLFITGP DVVKSVTNED VTQEELGGAK THTTMSGVAH RAFENDVDAL CNLRDFFNYL PLSSQDPAPV RECHDPSDRL VPELDTIVPL ESTKAYNMVD IIHSVVDERE FFEIMPNYAK NIIVGFARMN GRTVGIVGNQ PKVASGCLDI NSSVKGARFV RFCDAFNIPL ITFVDVPGFL PGTAQEYGGI IRHGAKLLYA FAEATVPKVT VITRKAYGGA YDVMSSKHLC GDTNYAWPTA EIAVMGAKGA VEIIFKGHEN VEAAQAEYIE KFANPFPAAV RGFVDDIIQP SSTRARICCD LDVLASKKVQ RPWRKHANIP L. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA. Reference: n/a. Function: nan