Propionate kinase (tdcD), Escherichia coli O6:H1, recombinant

Organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC). Source: E.coli. Expression Region: 1-402aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MNEFPVVLVI NCGSSSIKFS VLNASDCEVL MSGIADGINS ENAFLSVNGG EPAPLAHHSY EGALKAIAFE LEKRNLNDNV ALIGHRIAHG GSIFTESAII TDEVIDNIRR VSPLAPLHNY ANLSGIESAQ QLFPGVTQVA VFDTSFHQTM APEAYLYGLP WKYYEELGVR RYGFHGTSHR YVSQRAHSLL NLAEDDSGLV VAHLGNGASI CAVRNGQSVD TSMGMTPLEG LMMGTRSGDV DFGAMSWVAS QTNQSLGDLE RVVNKESGLL GISGLSSDLR VLEKAWHEGH ERAQLAIKTF VHRIARHIAG HAASLHRLDG IIFTGGIGEN SSLIRRLVME HLAVLGVEID TEMNNRSNSF GERIVSSENA RVICAVIPTN EEKMIALDAI HLGKVNAPAE FA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the conversion of propionyl phosphate and ADP to propionate and ATP. Reference: "Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002). Function: Catalyzes the conversion of propionyl phosphate and ADP to propionate and ATP.