Properdin (Cfp), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 23-464aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SDPVLCFTQY EESSGRCKGL LGRDIRVEDC CLNAAYAFQE HDGGLCQACR SPQWSAWSLW GPCSVTCSEG SQLRHRRCVG RGGQCSENVA PGTLEWQLQA CEDQPCCPEM GGWSEWGPWG PCSVTCSKGT QIRQRVCDNP APKCGGHCPG EAQQSQACDT QKTCPTHGAW ASWGPWSPCS GSCLGGAQEP KETRSRSCSA PAPSHQPPGK PCSGPAYEHK ACSGLPPCPV AGGWGPWSPL SPCSVTCGLG QTLEQRTCDH PAPRHGGPFC AGDATRNQMC NKAVPCPVNG EWEAWGKWSD CSRLRMSINC EGTPGQQSRS RSCGGRKFNG KPCAGKLQDI RHCYNIHNCI MKGSWSQWST WSLCTPPCSP NATRVRQRLC TPLLPKYPPT VSMVEGQGEK NVTFWGTPRP LCEALQGQKL VVEEKRSCLH VPVCKDPEEK KP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Reference: "Properdin, the terminal complement components, thrombospondin and the circumsporozoite protein of malaria parasites contain similar sequence motifs."Goundis A., Reid K.B.M.Nature 335:82-85(1988). Function: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.