Prominin-1 (Prom1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 509-794aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6XHis-SUMO-tagged. Target Protein Sequence: GANVEKLLCE PYENKKLLQV LDTPYLLKEQ WQFYLSGMLF NNPDINMTFE QVYRDCKRGR GIYAAFQLEN VVNVSDHFNI DQISENINTE LENLNVNIDS IELLDNTGRK SLEDFAHSGI DTIDYSTYLK ETEKSPTEVN LLTFASTLEA KANQLPEGKP KQAFLLDVQN IRAIHQHLLP PVQQSLNTLR QSVWTLQQTS NKLPEKVKKI LASLDSVQHF LTNNVSLIVI GETKKFGKTI LGYFEHYLHW VFYAITEKMT SCKPMATAMD SAVNGILCGY VADPLN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Reference: "Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells." Weigmann A., Corbeil D., Hellwig A., Huttner W.B. Proc. Natl. Acad. Sci. U.S.A. 94:12425-12430(1997). Function: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis