Prolactin receptor (PRLR), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 25-622aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: QLPPGKPEIF KCRSPNKETF TCWWRPGTDG GLPTNYSLTY HREGETLMHE CPDYITGGPN SCHFGKQYTS MWRTYIMMVN ATNQMGSSFS DELYVDVTYI VQPDPPLELA VEVKQPEDRK PYLWIKWSPP TLIDLKTGWF TLLYEIRLKP EKAAEWEIHF AGQQTEFKIL SLHPGQKYLV QVRCKPDHGY WSAWSPATFI QIPSDFTMND TTVWISVAVL SAVICLIIVW AVALKGYSMV TCIFPPVPGP KIKGFDAHLL EKGKSEELLS ALGCQDFPPT SDYEDLLVEY LEVDDSEDQH LMSVHSKEHP SQGMKPTYLD PDTDSGRGSC DSPSLLSEKC EEPQANPSTF YDPEVIEKPE NPETTHTWDP QCISMEGKIP YFHAGGSKCS TWPLPQPSQH NPRSSYHNIT DVCELAVGPA GAPATLLNEA GKDALKSSQT IKSREEGKAT QQREVESFHS ETDQDTPWLL PQEKTPFGSA KPLDYVEIHK VNKDGALSLL PKQRENSGKP KKPGTPENNK EYAKVSGVMD NNILVLVPDP HAKNVACFEE SAKEAPPSLE QNQAEKALAN FTATSSKCRL QLGGLDYLDP ACFTHSFH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. Reference: "Alternative splicing to exon 11 of human prolactin receptor gene results in multiple isoforms including a secreted prolactin-binding protein." Trott J.F., Hovey R.C., Koduri S., Vonderhaar B.K. J. Mol. Endocrinol. 30:31-47(2003). Function: This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.