Progesterone-induced-blocking factor 1 (PIBF1), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-757aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MSRKISKESK KVNISSSLES EDISLETTVP TDDISSSEER EGKVRITRQL IERKELLHNI QLLKIELSQK TMMIDNLKVD YLTKIEELEE KLNDALHQKQ LLTLRLDNQL AFQQKDASKY QELMKQEMET ILLRQKQLEE TNLQLREKAG DVRRNLRDFE LTEEQYIKLK AFPEDQLSIP EYVSVRFYEL VNPLRKEICE LQVKKNILAE ELSTNKNQLK QLTETYEEDR KNYSEVQIRC QRLALELADT KQLIQQGDYR QENYDKVKSE RDALEQEVIE LRRKHEILEA SHMIQTKERS ELSKEVVTLE QTVTLLQKDK EYLNRQNMEL SVRCAHEEDR LERLQAQLEE SKKAREEMYE KYVASRDHYK TEYENKLHDE LEQIRLKTNQ EIDQLRNASR EMYERENRNL REARDNAVAE KERAVMAEKD ALEKHDQLLD RYRELQLSTE SKVTEFLHQS KLKSFESERV QLLQEETARN LTQCQLECEK YQKKLEVLTK EFYSLQASSE KRITELQAQN SEHQARLDIY EKLEKELDEI IMQTAEIENE DEAERVLFSY GYGANVPTTA KRRLKQSVHL ARRVLQLEKQ NSLILKDLEH RKDQVTQLSQ ELDRANSLLN QTQQPYRYLI ESVRQRDSKI DSLTESIAQL EKDVSNLNKE KSALLQTKNQ MALDLEQLLN HREELAAMKQ ILVKMHSKHS ENSLLLTKTE PKHVTENQKS KTLNVPKEHE DNIFTPKPTL FTKKEAPEWS KKQKMKT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity. Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation. Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2. Isoform 4: The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect. Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein. Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004). Function: Isoform 1