Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2), recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 26-737aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: ADSEKPSSIP TDKLLVITVA TKESDGFHRF MQSAKYFNYT VKVLGQGEEW RGGDGINSIG GGQKVRLMKE VMEHYADQDD LVVMFTECFD VIFAGGPEEV LKKFQKANHK VVFAADGILW PDKRLADKYP VVHIGKRYLN SGGFIGYAPY VNRIVQQWNL QDNDDDQLFY TKVYIDPLKR EAINITLDHK CKIFQTLNGA VDEVVLKFEN GKARAKNTFY ETLPVAINGN GPTKILLNYF GNYVPNSWTQ DNGCTLCEFD TVDLSAVDVH PNVSIGVFIE QPTPFLPRFL DILLTLDYPK EALKLFIHNK EVYHEKDIKV FFDKAKHEIK TIKIVGPEEN LSQAEARNMG MDFCRQDEKC DYYFSVDADV VLTNPRTLKI LIEQNRKIIA PLVTRHGKLW SNFWGALSPD GYYARSEDYV DIVQGNRVGV WNVPYMANVY LIKGKTLRSE MNERNYFVRD KLDPDMALCR NAREMGVFMY ISNRHEFGRL LSTANYNTSH YNNDLWQIFE NPVDWKEKYI NRDYSKIFTE NIVEQPCPDV FWFPIFSEKA CDELVEEMEH YGKWSGGKHH DSRISGGYEN VPTDDIHMKQ VDLENVWLHF IREFIAPVTL KVFAGYYTKG FALLNFVVKY SPERQRSLRP HHDASTFTIN IALNNVGEDF QGGGCKFLRY NCSIESPRKG WSFMHPGRLT HLHEGLPVKN GTRYIAVSFI DP. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Reference: n/a. Function: nan