Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 (KAE1), Saccharomyces cerevisiae,

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 1-386aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MVNLNTIPPK NGRDYYIALG LEGSANKLGV GIVKHPLLPK HANSDLSYDC EAEMLSNIRD TYVTPPGEGF LPRDTARHHR NWCIRLIKQA LAEADIKSPT LDIDVICFTK GPGMGAPLHS VVIAARTCSL LWDVPLVGVN HCIGHIEMGR EITKAQNPVV LYVSGGNTQV IAYSEKRYRI FGETLDIAIG NCLDRFARTL KIPNEPSPGY NIEQLAKKAP HKENLVELPY TVKGMDLSMS GILASIDLLA KDLFKGNKKN KILFDKTTGE QKVTVEDLCY SLQENLFAML VEITERAMAH VNSNQVLIVG GVGCNVRLQE MMAQMCKDRA NGQVHATDNR FCIDNGVMIA QAGLLEYRMG GIVKDFSETV VTQKFRTDEV YAAWRD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. KAE1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Reference: "Sequencing and comparison of yeast species to identify genes and regulatory elements."Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.Nature 423:241-254(2003). Function: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. KAE1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.