Probable serine carboxypeptidase CPVL (CPVL), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 23-476aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: LFRSLYRSVS MPPKGDSGQP LFLTPYIEAG KIQKGRELSL VGPFPGLNMK SYAGFLTVNK TYNSNLFFWF FPAQIQPEDA PVVLWLQGGP GGSSMFGLFV EHGPYVVTSN MTLRDRDFPW TTTLSMLYID NPVGTGFSFT DDTHGYAVNE DDVARDLYSA LIQFFQIFPE YKNNDFYVTG ESYAGKYVPA IAHLIHSLNP VREVKINLNG IAIGDGYSDP ESIIGGYAEF LYQIGLLDEK QKKYFQKQCH ECIEHIRKQN WFEAFEILDK LLDGDLTSDP SYFQNVTGCS NYYNFLRCTE PEDQLYYVKF LSLPEVRQAI HVGNQTFNDG TIVEKYLRED TVQSVKPWLT EIMNNYKVLI YNGQLDIIVA AALTERSLMG MDWKGSQEYK KAEKKVWKIF KSDSEVAGYI RQAGDFHQVI IRGGGHILPY DQPLRAFDMI NRFIYGKGWD PYVG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for antigen presentation. Reference: Cloning and characterization of CPVL, a novel serine carboxypeptidase, from human macrophages.Mahoney J.A., Ntolosi B., DaSilva R.P., Gordon S., McKnight A.J.Genomics 72:243-251(2001). Function: May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for antigen presentation.