PRL-3, Recombinant Human (Protein tyrosine phosphatase type IVA 3)

Protein tyrosine phosphatase type IVA 3 (PRL-3), also known as PTP4A3, belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) that remove phosphate modifications from tyrosine residues on proteins. This protein enhances cell proliferation, cell motility and invasive activity. High levels of PRL-3 expression are associated with tumorigenesis and metastasis, thus it is overexpressed in metastatic colorectal, ovarian, liver and skin cancers. Description: His-tagged recombinant human PRL-3 (amino acids 1-173, UniProt Accession #O75365) expressed in E. coli, Molecular Weight: 21.6kDa (193 amino acids), confirmed by MALDI-TOF, AA Sequence: MGSSHHHHHH SSGLVPRGSH MARMNRPAPV EVSYKHMRFL ITHNPTNATL STFIEDLKKY GATTVVRVCE VTYDKTPLEK DGITVVDWPF DDGAPPPGKV VEDWLSLVKA KFCEAPGSCV AVHCVAGLGR APVLVALALI ESGMKYEDAI QFIRQKRRGA INSKQLTYLE KYRPKQRLRF, KDPHTHKTRC CVM, Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein, Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.