Potassium channel subfamily K member 13 (KCNK13), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-408aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MAGRGFSWGP GHLNEDNARF LLLAALIVLY LLGGAAVFSA LELAHERQAK QRWEERLANF SRGHNLSRDE LRGFLRHYEE ATRAGIRVDN VRPRWDFTGA FYFVGTVVST IGFGMTTPAT VGGKIFLIFY GLVGCSSTIL FFNLFLERLI TIIAYIMKSC HQRQLRRRGA LPQESLKDAG QCEVDSLAGW KPSVYYVMLI LCTASILISC CASAMYTPIE GWSYFDSLYF CFVAFSTIGF GDLVSSQNAH YESQGLYRFA NFVFILMGVC CIYSLFNVIS ILIKQSLNWI LRKMDSGCCP QCQRGLLRSR RNVVMPGSVR NRCNISIETD GVAESDTDGR RLSGEMISMK DLLAANKASL AILQKQLSEM ANGCPHQTST LARDNEFSGG VGAFAIMNNR LAETSGDR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Potassium channel displaying weak inward rectification in symmetrical K(+) solution. Reference: "Biophysical, pharmacological, and functional characteristics of cloned and native mammalian two-pore domain K+ channels." Lotshaw D.P. Cell Biochem Biophys 47:209-256(2007). Function: nan