Poly alpha-guluronate lyase 7A, Zobellia galactanivorans

Poly alpha-guluronate lyase 7A (ZgAly7A), assigned the E.C. number 4.2.2.11, is a derivative of Zobellia galactanivorans. It is an enzyme that participates in the eliminative cleavage of polysaccharides containing a terminal alpha-guluronate group, to give oligosaccharides with 4-deoxy-alpha-erythro-hex-4-enuronosyl groups at their non-reducing ends. The recombinant ZgAly7A, purified from Escherichia coli, is a single-domain Pectate Lyase family 7 (PL7) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Poly-alpha-guluronate lyases. Family: PL7. Ec Number: 4.2.2.11. Organism: Zobellia galactanivorans. Architecture: PL7. Optimal pH: 7. Optimal Temperature: 30°C. Substrates: sodium alginate