Pneumolysin (ply), recombinant Streptococcus pneumoniae

Organism: Streptococcus pneumoniae (strain ATCC BAA-255 / R6). Source: Yeast. Expression Region: 2-471aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: ANKAVNDFIL AMNYDKKKLL THQGESIENR FIKEGNQLPD EFVVIERKKR SLSTNTSDIS VTATNDSRLY PGALLVVDET LLENNPTLLA VDRAPMTYSI DLPGLASSDS FLQVEDPSNS SVRGAVNDLL AKWHQDYGQV NNVPARMQYE KITAHSMEQL KVKFGSDFEK TGNSLDIDFN SVHSGEKQIQ IVNFKQIYYT VSVDAVKNPG DVFQDTVTVE DLKQRGISAE RPLVYISSVA YGRQVYLKLE TTSKSDEVEA AFEALIKGVK VAPQTEWKQI LDNTEVKAVI LGGDPSSGAR VVTGKVDMVE DLIQEGSRFT ADHPGLPISY TTSFLRDNVV ATFQNSTDYV ETKVTAYRNG DLLLDHSGAY VAQYYITWDE LSYDHQGKEV LTPKAWDRNG QDLTAHFTTS IPLKGNVRNL SVKIRECTGL AWEWWRTVYE KTDLPLVRKR TISIWGTTLY PQVEDKVEND. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation, cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. Reference: n/a. Function: nan