Plexin-B2 (PLXNB2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 20-466aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: LRPRKLDFFR SEKELNHLAV DEASGVVYLG AVNALYQLDA KLQLEQQVAT GPALDNKKCT PPIEASQCHE AEMTDNVNQL LLLDPPRKRL VECGSLFKGI CALRALSNIS LRLFYEDGSG EKSFVASNDE GVATVGLVSS TGPGGDRVLF VGKGNGPHDN GIIVSTRLLD RTDSREAFEA YTDHATYKAG YLSTNTQQFV AAFEDGPYVF FVFNQQDKHP ARNRTLLARM CREDPNYYSY LEMDLQCRDP DIHAAAFGTC LAASVAAPGS GRVLYAVFSR DSRSSGGPGA GLCLFPLDKV HAKMEANRNA CYTGTREARD IFYKPFHGDI QCGGHAPGSS KSFPCGSEHL PYPLGSRDGL RGTAVLQRGG LNLTAVTVAA ENNHTVAFLG TSDGRILKVY LTPDGTSSEY DSILVEINKR VKRDLVLSGD LGSLYAMTQD KVFRLPV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496). Reference: "A dual binding mode for RhoGTPases in plexin signalling." Bell C.H., Aricescu A.R., Jones E.Y., Siebold C. PLoS Biol. 9:E1001134-E1001134(2011). Function: nan