Plexin-B1 (PLXNB1), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 20-535aa. Protein Length: Partial. Tag Info: N-terminal mFc-tagged. Target Protein Sequence: LQPLPPTAFT PNGTYLQHLA RDPTSGTLYL GATNFLFQLS PGLQLEATVS TGPVLDSRDC LPPVMPDECP QAQPTNNPNQ LLLVSPGALV VCGSVHQGVC EQRRLGQLEQ LLLRPERPGD TQYVAANDPA VSTVGLVAQG LAGEPLLFVG RGYTSRGVGG GIPPITTRAL WPPDPQAAFS YEETAKLAVG RLSEYSHHFV SAFARGASAY FLFLRRDLQA QSRAFRAYVS RVCLRDQHYY SYVELPLACE GGRYGLIQAA AVATSREVAH GEVLFAAFSS AAPPTVGRPP SAAAGASGAS ALCAFPLDEV DRLANRTRDA CYTREGRAED GTEVAYIEYD VNSDCAQLPV DTLDAYPCGS DHTPSPMASR VPLEATPILE WPGIQLTAVA VTMEDGHTIA FLGDSQGQLH RVYLGPGSDG HPYSTQSIQQ GSAVSRDLTF DGTFEHLYVM TQSTLLKVPV ASCAQHLDCA SCLAHRDPYC GWCVLLGRCS RRSECSRGQG PEQWLWSFQP ELGCLQ. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D (CSB-MP835707HUd9) at 5 µg/mL can bind human PLXNB1, the EC50 is 0.8179-1.357 µg/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496). Reference: n/a. Function: nan