Pleckstrin homology-like domain family A member 1 (PHLDA1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-401aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MRRAPAAERL LELGFPPRCG RQEPPFPLGV TRGWGRWPIQ KRREGARPVP FSERSQEDGR GPAARSSGTL WRIRTRLSLC RDPEPPPPLC LLRVSLLCAL RAGGRGSRWG EDGARLLLLP PARAAGNGEA EPSGGPSYAG RMLESSGCKA LKEGVLEKRS DGLLQLWKKK CCILTEEGLL LIPPKQLQHQ QQQQQQQQQQ QQQQPGQGPA EPSQPSGPAV ASLEPPVKLK ELHFSNMKTV DCVERKGKYM YFTVVMAEGK EIDFRCPQDQ GWNAEITLQM VQYKNRQAIL AVKSTRQKQQ HLVQQQPPSQ PQPQPQLQPQ PQPQPQPQPQ PQSQPQPQPQ PKPQPQQLHP YPHPHPHPHS HPHSHPHPHP HPHPHQIPHP HPQPHSQPHG HRLLRSTSNS A. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation. Reference: "Assignment of the human PHLDA1 gene to chromosome 12q15 by radiation hybrid mapping." Kuske M.D., Johnson J.P. Cytogenet. Cell Genet. 89:1-1(2000). Function: Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.