Platelet glycoprotein VI (Gp6), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 22-265aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: QSGPLPKPSL QAQPSSLVPL GQSVILRCQG PPDVDLYRLE KLKPEKYEDQ DFLFIPTMER SNAGRYRCSY QNGSHWSLPS DQLELIATGV YAKPSLSAHP SSAVPQGRDV TLKCQSPYSF DEFVLYKEGD TGSYKRPEKW YRANFPIITV TAAHSGTYRC YSFSSSSPYL WSAPSDPLVL VVTGLSATPS QVPTEESFPV TESSRRPSIL PTNKISTTEK PMNITASPEG LSPPFGFAHQ HYAK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely FYN or LYN) and SYK, the adapter protein LAT and leads to the activation of PLCG2. Reference: "The glycoprotein VI-phospholipase Cgamma2 signaling pathway controls thrombus formation induced by collagen and tissue factor in vitro and in vivo." Munnix I.C., Strehl A., Kuijpers M.J., Auger J.M., van der Meijden P.E., van Zandvoort M.A., oude Egbrink M.G., Nieswandt B., Heemskerk J.W. Arterioscler. Thromb. Vasc. Biol. 25:2673-2678(2005). Function: Collagen receptor involved in collagen-induced platelet adhesion and activation