Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-494aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MDSGYVEDSE ESSSEWPWRR GSQERRGHRR PGQKFIRIYK LFKSTSQLVL RRDSRSLEGS SDTALPLRRA GSLCSPLDEP VSPPSRAQRS RSLPQPKLGT QLPSWLLAPA SRPQRRRPFL SGDEDPKAST LRVVVFGSDR ISGKVARAYS NLRRLENNRP LLTRFFKLQF FYVPVKRSHG TSPGACPPPR SQTPSPPTDS PRHASPGELG TTPWEESTND ISHYLGMLDP WYERNVLGLM HLPPEVLCQQ SLKAEAQALE GSPTQLPILA DMLLYYCRFA ARPVLLQVYQ TELTFITGEK TTEIFIHSLE LGHSAATRAI KASGPGSKRL GIDGDREAVP LTLQIIYSKG AISGRSRWSN LEKVCTSVNL NKACRKQEEL DSSMEALTLN LTEVVKRQNS KSKKGFNQIS TSQIKVDKVQ IIGSNSCPFA VCLDQDERKI LQSVVRCEVS PCYKPEKSDL SSPPQTPPDL PAQAAPDLCS LLCLPIMTFS GALP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma mbrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG . Reference: "Large-scale proteomics analysis of the human kinome."Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.Mol. Cell. Proteomics 8:1751-1764(2009). Function: Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG (By similarity).